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multilocularis larvae within the intermediate host can be mimicked

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Сообщение  jy9202 в Пн Мар 31, 2014 12:59 pm

The expression of proteins was detected using primary antibody against GOLPH3, PARP, and B actin, The signal was de tected by the ECL Western blot detection kit, Band intensity was quantified using NIH Scion Image supplier JNJ-7706621 software and normalized to B actin. Statistical analysis Statistical analysis was performed using SPSS software, Differences in mRNA expression between tumor samples and the matched adjacent noncancerous tissue samples were evaluated using paired sample t tests. Pearsons chi square and Fishers exact tests were used to analyse the correlation between GOLPH3 expression and clinico pathological characteristics. Survival was determined using the Kaplan Meier methods and compared with the log rank test. Univariate and multivariate survival analyses were performed using the Cox proportional hazard regression model.<br><br> Two sided values of P 0. 05 were considered statistically significant. Results GOLPH3 expression and association with clinicopathological characteristics in CRC In this study, we examined GOLPH3 mRNA levels in matched cancerous and adjacent noncancerous colo 価格 LDN193189 rectal tissues from 30 CRC patients. qRT PCR results showed that GOLPH3 transcripts were significantly ele vated in cancerous tissues than in matched adjacent noncancerous tissues, Moreover, we measured GOLPH3 protein expression by immu nohistochemistry in 130 cases. Consistent with mRNA expression, high GOLPH3 expression was detected in 56 out of 130 CRC tissues, compared with 10 out of 75 in matched adjacent noncancerous tissues, Then we investigated possible correlations between GOLPH3 expression and CRC clinicalpathological characteristics.<br><br> Our data showed that GOLPH3 expression was correlated with gender, recurrence and survival, However, no significant associations were buy LY2228820 found between GOLPH3 expression and other clini copathological features, Association between GOLPH3 protein expression and survival of patients treated with 5 FU based adjuvant chemotherapy Kaplan Meier survival curves and log rank test survival analysis were used to determine the prognostic value of GOLPH3 on survival of patients who received 5 FU based adjuvant chemotherapy. The results showed that patients with tumors exhibiting high GOLPH3 expres sion had substantially longer disease free survival and overall survival than those with tumors exhibiting low GOLPH3 expression.<br><br> These data suggest that high GOLPH3 expression is associated with favourable progno sis in patients treated with 5 FU adjuvant chemotherapy. Furthermore, Cox proportional hazard regression analysis was performed to evaluate the prognostic sig nificance of clinicopathological parameters. Univariate analysis showed that high GOLPH3 expression was significantly associated with longer DFS and OS in patients treated with 5 FU based adjuvant chemotherapy. All factors that showed prognostic sig nificance in the univariate analysis were included in the multivariate analysis.


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